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Phenotypic, genetic and symbiotic characterization of Erythrina velutina rhizobia from Caatinga dry forest BJM
Rodrigues,Dalila Ribeiro; Silva,Aleksandro Ferreira da; Cavalcanti,Maria Idaline Pessoa; Escobar,Indra Elena Costa; Fraiz,Ana Carla Resende; Ribeiro,Paula Rose de Almeida; Ferreira Neto,Reginaldo Alves; Freitas,Ana Dolores Santiago de; Fernandes-Júnior,Paulo Ivan.
Abstract Erythrina velutina ("mulungu") is a legume tree from Caatinga that associates with rhizobia but the diversity and symbiotic ability of "mulungu" rhizobia are poorly understood. The aim of this study was to characterize "mulungu" rhizobia from Caatinga. Bacteria were obteined from Serra Talhada and Caruaru in Caatinga under natural regeneration. The bacteria were evaluated to the amplification of nifH and nodC and to metabolic characteristics. Ten selected bacteria identified by 16S rRNA sequences. They were tested in vitro to NaCl and temperature tolerance, auxin production and calcium phosphate solubilization. The symbiotic ability were assessed in an greenhouse experiment. A total of 32 bacteria were obtained and 17 amplified both symbiotic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biological nitrogen fixation; "Mulungu"; Diversity; Inoculant; 16S rRNA.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300503
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Symbiotic and agronomic efficiency of new cowpea rhizobia from Brazilian Semi-Arid Bragantia
Marinho,Rita de Cássia Nunes; Ferreira,Linnajara de Vasconcelos Martins; Silva,Aleksandro Ferreira da; Martins,Lindete Míria Vieira; Nóbrega,Rafaela Simão Abrahão; Fernandes-Júnior,Paulo Ivan.
ABSTRACT Cowpea is a very important crop to Brazilian Semi-Arid mainly small family-based farmers. Rhizobia inoculation is a practice, easy to use, and cheap technology that increases cowpea productivity. The aim of this study was to evaluate the efficiency of two new rhizobia isolates in greenhouse and field as well as classify them taxonomically. To bacterial identification the 16S rRNA gene of ESA 17 and ESA 18 isolates were sequenced. The greenhouse test was conducted with pots containing 3 L of soil and the bacterial isolates evaluated were ESA 17, ESA 18, BR 3267 or BR 3262 strains. A field experiment was implemented on a Vertisol in Juazeiro, Bahia State, to evaluate the cowpea growth and productivity. In this experiment, the peat-based inoculants...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bradyrhizobium; Inoculant; Biological nitrogen fixation; Strain selection; São Francisco River Valley.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052017000200273
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THE RESURRECTION PLANT TRIPOGON SPICATUS (POACEAE) HARBORS A DIVERSITY OF PLANT GROWTH PROMOTING BACTERIA IN NORTHEASTERN BRAZILIAN CAATINGA Rev. Bras. Ciênc. Solo
Fernandes-Júnior,Paulo Ivan; Aidar,Saulo de Tarso; Morgante,Carolina Vianna; Gava,Carlos Alberto Tuão; Zilli,Jerri Édson; Souza,Layane Silva Barbosa de; Marinho,Rita de Cássia Nunes; Nóbrega,Rafaela Simão Abrahão; Brasil,Marivaine da Silva; Seido,Sirando Lima; Martins,Lindete Míria Vieira.
Plant species that naturally occur in the Brazilian Caatinga(xeric shrubland) adapt in several ways to these harsh conditions, and that can be exploited to increase crop production. Among the strategic adaptations to confront low water availability, desiccation tolerance stands out. Up to now, the association of those species with beneficial soil microorganisms is not well understood. The aim of this study was to characterize Tripogon spicatusdiazotrophic bacterial isolates from the Caatingabiome and evaluate their ability to promote plant growth in rice. Sixteen bacterial isolates were studied in regard to their taxonomic position by partial sequencing of the 16S rRNA gene, putative diazotrophic capacity, in vitro indole-acetic acid (IAA) production and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biological nitrogen fixation; Inoculant; Diazotrophic bacteria; Semi-arid; Desiccation tolerant plants.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832015000400993
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